Synthesis, Characterization and Antitumor Activity of cis-bis(acylthioureato) platinum(II) Complexes, cis-[PtL2] [HL1=N,N-Diphenyl-N'-Benzoylthiourea or HL2=N,N-diphenyl-N'-(p-nitrobenzoyl)thiourea]

摘要

A low-molecular weight chromium-containing fraction of the material resulting from dichromatereduction by bovine liver homogenate was investigated by NMR and ES-MS. The ES-MS spectrum showed areadily detectable peak at m/z 786.1. The same molecular weight reasonably agreed with the relatively lowdiffusion coefficient measured by NMR-DOSY experiments on the main species observed in the 1H NMRspectrum. At least two downfield shifted and broad paramagnetic signals were apparent in the 1H NMRspectrum. Temperature dependence of chemical shift was exploited in order to estimate the diamagnetic shiftof the signals in the diamagnetic region of the spectrum. 2D TOCSY, NOESY, COSY and 1H-13C HMQCspectra revealed the presence of aromatic protons (which were assigned as His residues), Gly and some othershort chain amino-acids. Combinations of the molecular masses of such components together with acetate(which is present in the solution) and chromium atoms allowed a tentative proposal of a model for thecompound.